LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2342..2438) /label=attL2 /note="attL2 (c)" misc_signal complement(2453..2472) /note="T7 Promoter/priming site (c)" primer_bind 2480..2496 /note="M13 Reverse priming site" gene 2609..3418 /note="Kanamycin resistance gene" rep_origin 3539..4212 /note="pUC origin" vector join(2350..4215,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2335 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="MGC:184321 IMAGE:100016589" /clone_lib="NIH_MGC_428" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 with stop codon" source 692..2335 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 692..2335 /gene="GALNT10" /gene_synonym=DKFZp586H0623 /gene_synonym=GalNAcT10 /gene_synonym=pp-GalNAc-T10 /db_xref="GeneID:55568" /db_xref="HGNC:19873" /db_xref="MIM:608043" CDS 692..2335 /dnas_title="UDP-N-acetyl-alpha-D-galactosamine:polypeptid e N-acetylgalactosaminyltransferase 10 (GalNAc-T10)" /gene="GALNT10" /gene_synonym=DKFZp586H0623 /gene_synonym=GalNAcT10 /gene_synonym=pp-GalNAc-T10 /codon_start=1 /product="UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10)" /protein_id="AAI53182.1" /db_xref="GI:157169754" /db_xref="GeneID:55568" /db_xref="HGNC:19873" /db_xref="MIM:608043" /translation="MRRKEKRLLQAVALVLAALVLLPNVGLWALYRERQPDGTPGGSG AAVAPAAGQGSHSRQKKTFFLGDGQKLKDWHDKEAIRRDAQRVGNGEQGRPYPMTDAE RVDQAYRENGFNIYVSDKISLNRSLPDIRHPNCNSKRYLETLPNTSIIIPFHNEGWSS LLRTVHSVLNRSPPELVAEIVLVDDFSDREHLKKPLEDYMALFPSVRILRTKKREGLI RTRMLGASVATGDVITFLDSHCEANVNWLPPLLDRIARNRKTIVCPMIDVIDHDDFRY ETQAGDAMRGAFDWEMYYKRIPIPPELQKADPSDPFESPVMAGGLFAVDRKWFWELGG YDPGLEIWGGEQYEISFKVWMCGGRMEDIPCSRVGHIYRKYVPYKVPAGVSLARNLKR VAEVWMDEYAEYIYQRRPEYRHLSAGDVAVQKKLRSSLNCKSFKWFMTKIAWDLPKFY PPVEPPAAAWGEIRNVGTGLCADTKHGALGSPLRLEGCVRGRGEAAWNNMQVFTFTWR EDIRPGDPQHTKKFCFDAISHTSPVTLYDCHSMKGNQLWKYRKDKTLYHPVSGSCMDC SESDHRIFMNTCNPSSLTQQWLFEHTNSTVLEKFNRN" misc_feature 692..2335 /note="GALNT10 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccacagtcga caaaagaaaa cgtttttctt 721 gggagatggg cagaagctga aggactggca tgacaaggag gccatccgga gggacgctca 781 gcgcgtagga aatggagaac aaggaagacc ttaccccatg accgatgctg agagagtgga 841 tcaggcatac cgagaaaatg gatttaacat ctacgtcagt gataaaatct ccttgaatcg 901 ctctctccca gatatccggc acccaaactg caacagcaag cgctacctgg agacacttcc 961 caacacaagc atcatcatcc ccttccacaa cgagggctgg tcctccctcc tccgcaccgt 1021 ccacagtgtg ctcaatcgct cgcctccaga gctggtcgcc gagattgtac tggtcgacga 1081 cttcagtgat cgagagcacc tgaagaagcc tcttgaagac tacatggccc ttttccccag 1141 tgtgaggatt cttcgaacca agaaacggga agggctgata aggacccgaa tgctgggggc 1201 ctcagtggca actggggatg tcatcacatt cttggattca cactgtgaag ccaatgtcaa 1261 ctggcttccc cccttgcttg accgcattgc tcggaaccgc aagaccattg tgtgcccgat 1321 gattgatgta attgaccatg acgactttcg gtacgagaca caggcagggg atgccatgcg 1381 gggagccttt gactgggaga tgtactacaa gcggatcccg atccctccag aactgcagaa 1441 agctgacccc agcgacccat ttgagtctcc cgtgatggcc ggtggactgt tcgccgtgga 1501 tcggaagtgg ttctgggaac tcggcgggta tgacccaggc ttggagatct ggggagggga 1561 gcagtatgaa atctccttca aggtgtggat gtgtgggggc cgcatggagg acatcccctg 1621 ctccagggtg ggccatatct acaggaagta tgtgccctac aaggtcccgg ccggagtcag 1681 cctggcccgg aaccttaagc gggtggccga agtgtggatg gatgagtacg cagagtacat 1741 ttaccagcgc cggcctgaat accgccacct ctccgctggg gatgtcgcag tccagaaaaa 1801 gctccgcagc tcccttaact gcaagagttt caagtggttt atgacgaaga tagcctggga 1861 cctgcccaaa ttctacccac ccgtggagcc cccggctgca gcttgggggg agatccgaaa 1921 tgtgggcaca gggctgtgtg cagacacaaa gcacggggcc ttgggctccc cactaaggct 1981 agagggctgc gtccgaggcc gtggggaggc tgcctggaac aacatgcagg tattcacctt 2041 cacctggaga gaggacatcc ggcctggaga cccccagcac accaagaagt tctgctttga 2101 tgccatttcc cacaccagcc ctgtcacgct gtacgactgc cacagcatga agggcaacca 2161 gctgtggaaa taccgcaaag acaagaccct gtaccaccct gtcagtggca gctgcatgga 2221 ctgcagtgaa agtgaccata ggatcttcat gaacacctgc aacccatcct ctctcaccca 2281 gcagtggctg tttgaacaca ccaactcaac agtcttggaa aaattcaata ggaacTAGGA 2341 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 2401 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2461 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2521 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2581 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2641 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2701 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2761 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2821 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2881 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2941 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 3001 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 3061 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 3121 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 3181 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 3241 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 3301 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 3361 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3421 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3481 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3541 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3601 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3661 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3721 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3781 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3841 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3901 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3961 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 4021 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 4081 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 4141 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 4201 TTTTGCTCAC ATGTT //